Constitutively Activating EPOR

Authors

• Erin Anson, St. John Fisher University

Document Type

Poster Presentation

Publication Date

4-17-2026

Keywords

fsc2026

Abstract

Abstract: Erythropoiesis, the process of making red blood cells, depends on signals from erythropoietin (EPO). In the lab, HUDEP‑2 cells are widely used to study human red blood cell development, but they require expensive EPO to survive and mature. This project proposes two strategies to make these cells grow without added EPO by creating “always‑on” versions of the EPO receptor (EPOR) or its signaling partner JAK2. One approach would introduce mutated genes using plasmids to artificially boost signaling. The second approach would use CRISPR/Cas9 to place activating mutations directly into the cell’s own DNA, allowing the receptor to function without EPO. If successful, this work would create a more affordable and accessible cell model for studying conditions like polycythemia, where the body produces too many red blood cells.

Comments

Poster presented at the 2026 Fisher Showcase, St. John Fisher University, April 17, 2026.

Publication Information

Anson, Erin, "Constitutively Activating EPOR" (2026). Fisher Showcase 2026. Paper 69.
https://fisherpub.sjf.edu/fsc2026/69

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