Document Type
Article
Publication Date
6-2019
Department
Biology
Abstract
The presence of primordial germ cells (PGCs) is crucial for proper gonad formation in zebrafish (Danio rerio). The many aspects of PGC migration that allow these cells to reach the proper location at the gonadal ridge include receptors, ligands, germ plasm components, and internal maintenance of PGCs. Any one of these factors could be affected by endocrine-disrupting chemicals (EDCs), which have been shown to alter the directed migration of these cells during early embryonic development. Based on recent research wherein the EDC bisphenol A (BPA) inhibited normal PGC migration, we have used the same dose of BPA to determine the impact of BPA on a gene central to proper germ cell migration. Zebrafish embryos were exposed to BPA, and the levels of the target gene nanos-1 were analyzed using quantitative real-time PCR (q-PCR). The target gene nanos-1 is a critically important germplasm component that allows for survival and proper migration of PGCs. The q-PCR results showed that BPA did not affect the transcription level of nanos-1 in zebrafish embryos.
DOI
https://doi.org/10.33697/ajur.2019.012
Publication Information
Babich, Bridget; Roba, George; Safura, Siti Sarah; Callahan, Kevin; and Freeman, Edward A. (2019). "Transcription of nanos-1 in Zebrafish Embryos is not Affected by Bisphenol A: Evaluated Using Quantitative Real-Time PCR." American Journal of Undergraduate Research 16.1, 15-21.
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Comments
This article was originally published in the American Journal of Undergraduate Research, 2019, 16(1), pp.15-21. It can also be viewed on the publisher's website: https://doi.org/10.33697/ajur.2019.012