Measurement of Chiral Recognition in Cyclodextrins by Fluorescence Anisotropy

Document Type

Book Chapter

Publication Date

10-4-2006

Abstract

This chapter discusses the chiral recognition in CD, which can be described as the discrimination between the two enantiomers of a chiral molecule. Despite the subtle differences between enantiomers, the biological effects of chiral drugs can be radically distinct for each enantiomer. The section on measurement of chiral recognition discusses various processes in meaning accurately the phenomenon of chiral recognition. The polarization based spectroscopic technique that has been widely used to study molecular interactions, is discussed with mathematical methods and fluorescence anisotropy. The determination of thermodynamic binding parameters in CD is explained with examples and schematic illustrations. The results from experiments are provided with illustration for CD types and the determination of binaphthyl phosphate (BNP) are given in form of a table. The studies presented in this chapter clearly demonstrate the potential that exists for using fluorescent anisotropy to study chiral recognition in CD-based systems, which has ramifications for both fundamental and applied perspectives.

DOI

https://doi.org/10.1016/B978-044452780-6/50004-8

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