The composition of freshwater bacterial populations is affected by a wide variety of factors. Temperature, acidity, organic matter, and environmental pollutants like industrial chemicals and antibiotics are a few examples. The impact that bodies of freshwater have on human activity and the wider ecosystem warrants the systematic identification of microbial flora, and in particular of species known to be pathogenic in plants and animals. In order to achieve the long term goal of using satellite imagery to predict the occurrence of specific bacterial species, our team is in the process of creating a multi-location, multi-year microbial flora database for the Rochester Lake Ontario embayment and nearby bodies of water. Our collaborators at the Rochester Institute of Technology have provided us with water samples collected at these locations during the summers of 2013 and 2014. In this work we present and analyze data from said samples. Using 16S ribosomal DNA data we characterize bacterial populations, determine their geographical distribution, establish genera prevalence and discuss the presence of and investigate antibiotic resistance in several pathogenic species. The scope of our long term project and the summer of 2015- sample collection are also considered.
Zimmerman, Daniel; Moore, Laura; Dora, Joseph; Concha, J. A.; Raqueno, N.; Herman, Maryann; and Ontiveros, Fernando, "Multi-Year Analysis of Microbial Populations in the Rochester-Lake Ontario Embayment" (2015). Biology Faculty/Staff Publications. Paper 20.
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Presented at the Western NY American Society of Microbiology Regional Conference in Amherst, New York, May 20, 2015.