Gene delivery using chemical methods

Document Type

Book Chapter

Publication Date

5-2-2025

Abstract

In lieu of an abstract, here is the chapter's 1st paragraph:

The bottleneck of gene therapy is the development of a method by which a therapeutic gene can be delivered to a cell site where gene expression can be accomplished. The process of introducing a gene into cells for the purpose of gene expression is called transfection. A number of methods have been developed for transfecting eukaryotic cells. These can be classified as either viral or nonviral. The ideal method for gene delivery should fulfill three basic requirements: 1. Protect against enzymatic degradation in the intracellular environment. 2. Transport the transgene across the plasma membrane and into the nucleus of the target cell. 3. Be devoid of any immunogenic response.

Comments

Chapter published in Challenges in Delivery of Therapeutic Genomics and Proteomics, Academic press, May 2, 2025. Second edition.

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